Qihuang decoction promotes the recovery of intestinal immune barrier dysfunction after gastrectomy in rats.
Year: 2018
Type: article
Abstract: OBJECTIVE: This study aims to observe the effect of Qihuang decoction on small intestinal mucosal barrier after gastrectomy in rats. MATERIALS AND METHODS: A total of 80 Wistar rats were randomly divided into normal group, sham operation group, enteral nutrition group (EN) and Qihuang decoction group (EN+QH), there were 20 rats in each group. Both the EN group and the EN+QH group underwent gastrectomy. Instillation of enteral nutrition in the small intestine was performed after operation in the EN group. Instillation of enteral nutrition and Qihuang decoction in the small intestine was performed after operation in the EN+QH group. Only the abdominal incision and closing was performed in the sham operation group without drug and nutritional intervention. The expression levels of tight junction proteins in intestinal epithelial cells were determined by western blotting method. The sIgA content in different anatomic sites of intestinal mucosa was determined by double antibody-PEG radioimmunoassay technique. The number of IgA+B cells in different anatomic sites of intestinal mucosa was determined by immunohistochemical method. RESULTS: <0.05). CONCLUSIONS: Qihuang decoction can promote the proliferation and differentiation of IgA+B lymphocytes and increase the sIgA content in intestinal mucosal immune barrier after gastrectomy in rats, it also can promote the expression of tight junction proteins to improve the permeability of intestinal mucosa and promote the recovery of intestinal immune barrier dysfunction in rats after gastrectomy by inhibiting the tight junction associated proteins' phosphorylation induced by Rho/ROCK signaling pathway.
Source: PubMed
Authors: Hui Peng, Yi Shen, Qi Zhang, Juda Liu, Zhen Wang, Long Huang, Fuhai Zhou, Jian Yu, Mingyang Liu, Yiyang Yuan, Shushan Yu, Qingsheng Yu
Institution: Anhui University of Traditional Chinese Medicine
Language: English
Subfield: Nutrition and Dietetics
Field: Nursing
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2972364741