Year: 2019
Type: review
Abstract: As a "holy grail" of neuroscience, optical imaging of membrane potential could enable high resolution measurements of spiking and synaptic activity in neuronal populations. This has been partly achieved using organic voltage-sensitive dyes in vitro, or in invertebrate preparations yet unspecific staining has prevented single-cell resolution measurements from mammalian preparations in vivo. The development of genetically encoded voltage indicators (GEVIs) and chemogenetic sensors has enabled targeting voltage indicators to plasma membranes and selective neuronal populations. Here, we review recent advances in the design and use of genetic voltage indicators and discuss advantages and disadvantages of three classes of them. Although genetic voltage indicators could revolutionize neuroscience, there are still significant challenges, particularly two-photon performance. To overcome them may require cross-disciplinary collaborations, team effort, and sustained support by large-scale research initiatives.
Source: BMC Biology
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,785
Funders: National Science Foundation, Pew Charitable Trusts, National Institutes of Health, Multidisciplinary University Research Initiative, National Institute of Mental Health, National Eye Institute, National Institute of Neurological Disorders and Stroke, Army Research Office, Army Research Laboratory
Awards: G1242600127, G1480873338, G2321596477, G263973742, G2723399458, Meeting: Coordinating Global Brain Projects, Rockefeller University, September 19th, 2016, G4012432327, G5259331294, Imaging Functional Connectivity in Visual Cortex, CRCNS: Modeling the nanophysiology of dendritic spines, Functional connectomics of the neocortical microcircuit, G7452299184, Effect of GABAergic inhibition of dendritic spines on synaptic pruning in the medial prefrontal cortex during adolescence
OpenAlex ID: W2972388288