The effects of BPA and BPS on microRNAs during bovine oocyte maturation and early embryo development
Year: 2019
Type: dissertation
Abstract: This thesis investigates the effects of endocrine disrupting chemicals, Bisphenol A and Bisphenol S, on the expression of microRNAs during bovine oocyte maturation and early embryo development. Regulation of development involves crucial events that are uniquely susceptible to EDC interference that can inappropriately activate cellular pathways. While the resulting effects on fertility are probably the result of changes in canonical gene expression, evidence is emerging to suggest that small noncoding RNAs known as microRNAs may also be participants in the response to EDCs. This study hypothesized that EDCs alter expression of key miRNAs during development. To this end, miR-21, -155, -34c, -146a expression were examined in an in vitro bovine model of matured cumulus-oocyte complexes, fertilized embryos, and cultured cumulus cells treated with BPA or BPS. The experimental data reveals alterations in microRNA expression after BPA treatment but not in BPS treatment, suggesting that it may act differently than BPA.
Author: Reem Sabry
Language: English
Subfield: Health, Toxicology and Mutagenesis
Field: Environmental Science
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W2972840829