POTENTIAL OF Trichoderma viride AND Rhizobium leguminosarum IN COMBINATION WITH TOPSIN M70 FUNGICIDE FOR MANAGEMENT DAMPING-OFF DISEASE OF PEA PLANTS CAUSED BY Rhizoctonia solani
Year: 2018
Type: article
Abstract: Efficacies of Trichoderma viride, Rhizobium leguminosarum and the fungicideTopsin M70% individually and/or their mixtures were tested in vitro and greenhouse conditions tocontrol damping-off and root rot diseases of pea plants (Pisum sativum L., cv. Master P) caused byRhizoctonia solani. The ability of tested Rhizobium leguminosarum and Trichodermia viride to exhibitplant growth promoting rhizobacteria (PGPR)-properties including ability to solubilize-P andproduction of IAA, as well as production of siderophores, hydrocyanic acid (HCN) and secretion ofcell-wall degrading enzymes (chitinase and protease) were investigated. Also, under in vitro conditions theeffect of Topsin M70% on growth of R. solani and T. viride, R. leguminosarum and their mixtures wasdetermined. The fungicide effective concentration was found to range from 10 to 50 ppm for R. solaniand T. viride mycelial growth being 83.30, 100, 76.08 and 100% at 40 and 50 ppm of Topsin M70%.The same trend was obtained with R. leguminosarum that showed maximum tolerance at 40 and 50ppm of Topsin M70% with the average of 52.19 and 59.85% inhibition over control, respectively.Additionally, greenhouse conditions were conducted on sandy clay soil at Etay El Baroud AgriculturalResearch Station, Beheria Government, Egypt to study the singly effect of seed soaking in T. viride,R. leguminosarum or their mixtures with the soil drench fungicide in pots after sowing of pea cv.Master P in concern. Significant decrease of damping-off and root rot of pea was obtained. Numbersof survived plants, shoot length as well as, fresh and dry weight were recorded. The combinedtreatments R. leguminosarum + Topsin M70%, T. viride + Topsin M70% and R. leguminosarum +T. viride + Topsin M70% were the most effective ones resulting the least percentage of total dampingoffand the highest percentage of healthy plants being 96.67 and100.00%, respectively. Seed treatmentwith T. viride, R. leguminosarum and drenched fungicide individually or in mixture improved plantgrowth as indicated by the increased growth parameters and the physiological activities(phytosynthetic pigments, peroxidase and polyphenol oxidase), especially in combined treatments.Peroxidase and polyphenol oxidase activities were increased in the different treatments- even, thefungicide drench treatment. Reduction in total damping-off was positively correlated with bothperoxidase (R2 = 96.70, P ˂ 0.005) and polyphenol oxidase (R2 = 89.60, P ˂ 0.005) activities.
Authors: Gehad Abd El-Wahab, Soad El-Sayed
Institution: Agricultural Research Center
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W2974834655