[Effect of Naoluo Xintong on proliferation and differentiation of neural stem cells and β-tubulin Ⅲ/GFAP].
Year: 2019
Type: article
Abstract: OBJECTIVE: . METHODS: drug-containing serum group (NLXT group), and 10% Naoluoxintong drug-containing serum with inhibitor Y27632 group (Y-27632 group) with corresponding treatments. The activity of the NSCs was detected after the treatments using MTT assay, and the migration of the cells was observed with Transwell assay. The expressions of β-tubulin Ⅲ, GFAP and MAP-2 proteins in the cells were detected with immunoblotting, and the expressions of DCX, NEUN, and β-tubulin Ⅲ were also detected with immunofluorescence assay. RESULTS: < 0.05). The numbers of β-tubulinⅢ/ GFAP, BrdU/DCX, and BrdU/NEUN labeled cells in the NLXT group and Y-27632 group were significantly greater than those in the MC group. CONCLUSIONS: by regulating the expressions of β-tubulinⅢ/GFAP.
Source: Europe PMC (PubMed Central)
Authors: Ling He, Xiaoqian Shi, Wei Xu, Hui Tan, Jian Wang
Institution: Anhui University of Traditional Chinese Medicine
Language: English
Subfield: Developmental Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2976388690