Year: 1997
Type: dissertation
Abstract: Cardiac iron overload is the major cause of morbidity and mortality in iron-loading diseases. Iron-induced cardiac dysfunction can be mimicked in vitro using cultures of iron-loaded neonatal rat cardiac myocytes. In this study, the presence of the Iron Regulatory Protein (IRP) has been demonstrated in cardiac myocytes by mRNA expression and RNA-binding activity. Iron-induced changes in transferrin receptor mRNA expression and Iron Responsive Element (IRE)-IRP binding occurred as predicted by the IRP system of cellular iron control. In addition, an iron-induced dose-dependent increase in ferritin transcript levels and decrease in total IRP indicated that other iron-sensitive controls may exist in myocytes. Furthermore, increased GAPDH mRNA levels suggest that this transcript may be sensitive to changes in cellular iron status. Attaining a greater knowledge of iron regulation in cardiac myocytes will further our understanding of iron's toxicity in heart cells.
Author: Josephine Barbara Sirna
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2977416360