Work

Probing protein–protein and protein–substrate interactions in the dynamic membrane-associated ternary complex of cytochromes P450, b 5 , and reductase

Year: 2019

Type: article

Abstract: Cytochrome P450 (cytP450) interacts with two redox partners, cytP450 reductase and cytochrome-b 5 , to metabolize substrates. Using NMR, we reveal changes in the dynamic interplay when all three proteins are incorporated into lipids nanodiscs in the absence and presence of substrates.

Source: Chemical Communications

DOI: 10.1039/c9cc05904k

Authors: Katherine A. Gentry, G. M. Anantharamaiah, Ayyalusamy Ramamoorthy

Institutions: University of Michigan, Universitat Autònoma de Barcelona, University of Alabama at Birmingham Hospital, University of Alabama at Birmingham, UAB Medicine

Language: English

Topic: Lipid Membrane Structure and Behavior

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute on Aging, National Institute of General Medical Sciences, National Center for Research Resources