Spin∞: an updated miniaturized spinning bioreactor design for the generation of human cerebral organoids from pluripotent stem cells
Year: 2019
Type: article
Abstract: Three-dimensional (3D) brain organoids derived from human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), have become a powerful system to study early development events and to model human disease. Cerebral organoids are generally produced in static culture or in a culture vessel with active mixing, and the two most widely used systems for mixing are a large spinning flask and a miniaturized multi-well spinning bioreactor (also known as Spin Omega (SpinΩ)). The SpinΩ provides a system that is amenable to drug testing, has increased throughput and reproducibility, and utilizes less culture media. However, technical limitations of this system include poor stability of select components and an elevated risk of contamination due to the inability to sterilize the device preassembled. Here, we report a new design of the miniaturized bioreactor system, which we term Spinfinity (Spin∞) that overcomes these concerns to permit long-term experiments. This updated device is amenable to months-long (over 200 days) experiments without concern of unexpected malfunctions.
Source: HardwareX
Institutions: Vanderbilt University, Vanderbilt-Ingram Cancer Center
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $500
Funders: National Science Foundation, Vanderbilt University, Vanderbilt University Medical Center, Chan Zuckerberg Initiative, National Institutes of Health, National Institute on Aging, National Eye Institute, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Environmental Health Sciences
Awards: G1344704808, G1559243861, G2019238319, G2758917292, G2778149835, Engineering a Pro-Vasculogenic Capillary Network Regulating Host Responses, Vanderbilt Mouse Metabolic Phenotyping Center, Cancer Center Support Grant, G540534827, Eunice Kennedy Shriver Intellectual and Developmental Disabilities Research Center at Vanderbilt University, G725763919, G734120740, G9000002043, G9000060478, G9000106052, G9000159340, Vanderbilt Interdisciplinary Training Program in Alzheimer's Disease, Molecular and Cellular Basis for Digestive Diseases, Vanderbilt Diabetes Research Center, Core Grant in Vision Research, Molecular Principles Governing Mitochondrial and Peroxisomal Remodeling as Drivers of Cell Fate and Identity
OpenAlex ID: W2979512947