Work

Cytotoxicity of aqueous cigarette smoke extract is affected by properties of pipettes used to prepare the extract.

Year: 2019

Type: article

Abstract: studies, and the influence of different method parameters on CSE composition and toxicity remains incompletely understood. Herein, we prepared CSE by bubbling cigarette smoke through mammalian cell culture medium, varying the type of pipette inserted into the recipient medium. Changing this one component of the preparation apparatus had a marked effect on the toxicity of the resulting CSE. Since many other parameters can also be varied in CSE preparation, these results stress the importance of standardization within and between studies.

Source: PubMed

Authors: Eric D Bernstein, Zhaohao Liao, Kenneth W Witwer

Institutions: Johns Hopkins University, Johns Hopkins Medicine

Language: English

Topic: Smoking Behavior and Cessation

Subfield: Physiology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institute on Drug Abuse, National Institute of Allergy and Infectious Diseases

Awards: Extracellular Vesicle-Mediated Immunomodulation in HIV Infection and Nicotine Abuse, Extracellular Vesicle and Extracellular RNA Biomarkers of HIV-1 Central Nervous System Pathogenesis and Cigarette Use, The Johns Hopkins Center for AIDS Research (JHU CFAR)