Work

System configuration optimization for mesoscopic fluorescence molecular tomography

Year: 2019

Type: article

Abstract: conditions; however, these are increasingly challenging to image as they are typically a few millimeters thick and turbid, limiting the usefulness of classical fluorescence microscopic techniques. For such applications, we developed a Mesoscopic Fluorescence Molecular Tomography methodology that collects high information content data to enable high-resolution tomographic reconstruction of fluorescence biomarkers at millimeters depths. This imaging approach is based on an inverse problem; hence, its imaging performances are dependent on critical technical considerations including optode sampling, forward model design and inverse solver parameters. Herein, we investigate the impact of the optical system configuration parameters, including detector layout, number of detectors, combination of detector and source numbers, and scanning mode with uncoupled or coupled source and detector array, on the 3D imaging performances. Our results establish that an MFMT system with a 2D detection chain implemented in a de-scanned mode provides the optimal imaging reconstruction performances.

Source: Biomedical Optics Express

DOI: 10.1364/boe.10.005660

Authors: Fugang Yang, Denzel Faulkner, Ruoyang Yao, Mehmet S. Ozturk, Qinglan Qu, Xavier Intes

Institutions: Shandong Institute of Business and Technology, Rensselaer Polytechnic Institute, Yuhuangding Hospital, Affiliated Hospital of Qingdao University

Language: English

Topic: Optical Imaging and Spectroscopy Techniques

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

Open Access status: gold

Estimated APC paid: $1,913

Funders: National Natural Science Foundation of China, Natural Science Foundation of Shandong Province, National Institutes of Health, National Institute of Biomedical Imaging and Bioengineering