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Beneficial Effects of Angiotensin-(1–7) on CD34+ Cells From Patients With Heart Failure

Year: 2017
Type: article
Abstract: The dysfunctional nature of CD34 cells from patients with heart failure (HF) may make them unsuitable for autologous stem-cell therapy. In view of evidence that the vasoprotective axis of the renin-angiotensin system (RAS) improves CD34 cell functions, we hypothesized that CD34 cells from patients with HF will be dysfunctional and that angiotensin-(1-7) [Ang-(1-7)] would improve their function. Peripheral blood was collected from New York Heart Association class II-IV patients with HF (n = 31) and reference subjects (n = 16). CD34 cell numbers from patients with HF were reduced by 47% (P < 0.05) and also displayed 76% reduction in migratory capacity and 56% (P < 0.05) lower production of nitric oxide. These alterations were associated with increases in RAS genes angiotensin-converting enzyme and AT2R (595%, P < 0.05) mRNA levels and 80% and 85% decreases in angiotensin-converting enzyme 2 and Mas mRNA levels, respectively. Treatment with Ang-(1-7) enhanced CD34 cell function through increased migratory potential and nitric oxide production, and reduced reactive oxygen species generation. These data show that HF CD34 cells are dysfunctional, and Ang-(1-7) improves their functions. This suggests that activation of the vasoprotective axis of the RAS may hold therapeutic potential for autologous stem-cell therapy in patients with HF.
DOI: 10.1097/fjc.0000000000000556
Authors: Colleen T. Cole-Jeffrey, Carl J. Pepine, Michael J. Katovich, Maria B. Grant, Mohan K. Raizada, Sugata Hazra
Language: English
Field: Medicine
Open Access status: green
OpenAlex ID: W2980550619
FWCI: 0.623
Cites: 27
Cited by: 8
Related to: 10