Work

Deriving rabbit embryonic stem cells by small molecule inhibitors.

Year: 2019

Type: article

Abstract: by RT-PCR. Domed rbES showed positive staining for Rex1, Fgf4, Klf4, Nanog and Oct4 by immunofluorescence chemistry. Further deleting either one factor in 3i medium as CHIR99021, PD0325901, Gö6983 or bFGF resulted in disappearing of domed rbES colonies. The optimal concentrations of 3i contained 0.75 µM PD0325901, 2.25 µM CHIR99021, and 4.5 µM Gö6983. Our work, in combination of different inhibitors for deriving rabbit ES, supports that the network of signal pathways plays an important role in ES self-renew, propagation and maintenance, and sheds light on deriving authentic properties of rbES in an important yet understudied model animal species.

Source: Europe PMC (PubMed Central)

Authors: Jiao Liu, Xiumei Zhu, Jinshan Li, Zhihui Liu, Yanhong Liu, Fei Xue, Lan Yang, Liyou An, Chien-Hong Chen, Giorgio Antonio Presicce, Qiping Zheng, Fuliang Du

Institutions: Nanjing Normal University, Renova Life (United States), Jiangsu University

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2980761547

FWCI: 0.297

Cites: 48

Cited by: 5

Related to: 10