Phthalates and Phthalate Alternatives: Effects on Proliferative and Estrogenic Target Genes in Ishikawa Cells
Year: 2014
Type: article
Abstract: Phthalates are used as plasticizers in many of the products found in medical, household, and industrial applications. Much research has not been completed on the effects of these phthalates as potential endocrine disrupting chemicals (EDCs). As these chemicals are ingested, the mechanism by which they affect the reproductive system is largely unknown. The purpose of this study was to observe how 2 phthalates, Di-n-butyl phthalate (DBP) and Diisononyl phthalate (DINP), and 2 phthalate alternatives, Dioctyl terephthalate (DOTP) and BHT (butylated hydroxytoluene)affect uterine cells in comparison to a vehicle treatment and 17β-Estradiol treatment. Changes in expression of mRNA were observed using reverse transcription polymerase chain reaction. Results from this study show that based on trends of change in the genes CD1, C-myc, ERα, PR, and HOXA10, each of the four chemical treatments changed proliferation in Ishikawa cells. Our results have opened possible classifications for mechanisms that the chemical treatments may follow as potential EDCs.
Authors: Ranjani Sundar, Ping Yin, Serdar E. Bulun
Language: English
Subfield: Health, Toxicology and Mutagenesis
Field: Environmental Science
Domain: Physical Sciences
Open Access status: green
Funder: National Institutes of Health
OpenAlex ID: W298092555