Work

High-Frequency Raman Analysis in Biological Tissues Using Dual-Wavelength Excitation Raman Spectroscopy

Year: 2019

Type: article

Abstract: A dual-wavelength excitation Raman probe with laser inputs at 866 nm or 1064 nm is customized and integrated into a compact Raman spectrometer that is based on an InGaAs detector. Under 1064 nm illumination, the spectrometer detects fingerprint Raman signals below 2000 cm –1 . While under 866 nm illumination, the spectral range is extended to cover high-frequency region (2400–4000 cm –1 ) that includes major C–H and O–H Raman vibrations. We demonstrate that the dual excitation InGaAs Raman is beneficial in detecting high-frequency Raman signals, especially water contents in high-fluorescent biological samples such as human dental tissues, grape skin, and plum skin due to the suppressed fluorescence interference.

Source: Applied Spectroscopy

DOI: 10.1177/0003702819881762

Authors: Wencai He, Bolan Li, Shan Yang

Institution: Jackson State University

Language: English

Topic: Spectroscopy Techniques in Biomedical and Chemical Research

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institute of General Medical Sciences, National Institute of Dental and Craniofacial Research

Awards: G2805336796, G6703709948