Work

SiRNA silencing of VEGF, IGFs, and their receptors in human retinal microvascular endothelial cells.

Year: 2018

Type: article

Abstract: . IGF-I transfection with each VEGF isoform reduced sphere- forming and migration capacities with robust upregulation of caspase-9, COX-2, MAPK, PKC, and VEGF receptors. At 48 hours, the effects were reversed with a majority of genes downregulated, except with IGF-I and NP-1 transfection. Using RNA interference for targeted inhibition of VEGF isoforms in conjunction with IGF-I may be preferable for suppression of HREC overgrowth in vasoproliferative retinopathies such as ROP.

Source: Europe PMC (PubMed Central)

Authors: Yona Nicolau, Fayez Bany-Mohammed, Charles L Cai, Jacob V Aranda, Kay D Beharry

Institutions: University of California, Irvine, SUNY Downstate Health Sciences University, State University of New York

Language: English

Topic: Angiogenesis and VEGF in Cancer

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: Eunice Kennedy Shriver National Institute of Child Health and Human Development

Award: Molecular and Clinical Pharmacology of Retinopathy of Prematurity