Work

CRISPR-Induced TMPRSS2-ERG Gene Fusions in Mouse Prostate Organoids.

Year: 2017

Type: article

Abstract: TMPRSS2-ERG fusions are common genetic events in prostate cancer. Until now, this genetic alteration was modelled by ERG overexpression. In this short communication, we report the creation of mouse prostate organoids that have undergone gene fusion through a CRISPR/Cas9-based strategy. The genetic fusion of TMPRSS2 and ERG results in ERG overexpression. This effect is androgen receptor-mediated, as expression of the fusion transcript can be restored to wildtype ERG levels by treatment with the androgen receptor antagonist Nilutamide.

Source: PubMed

Authors: Else Driehuis, Hans Clevers

Institutions: Royal Netherlands Academy of Arts and Sciences, University Medical Center Utrecht, Princess Máxima Center

Language: English

Topic: Prostate Cancer Treatment and Research

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Cancer Institute

Award: (PQA-4) Organoid Omics To Detect And Defeat Ductal Pancreatic Cancer