Fiber typing human skeletal muscle with fluorescent immunohistochemistry
Year: 2019
Type: review
Abstract: Skeletal muscle myosin heavy chain (MyHC) fiber type composition is a critical determinant of overall muscle function and health. Various approaches interrogate fiber type at the single cell, but the two most commonly utilized are single-muscle fiber sodium dodecyl sulfate-polyacrylamide gel electrophoresis (smfSDS-PAGE) and fluorescent immunohistochemistry (IHC). Although smfSDS-PAGE is generally considered the “gold standard,” IHC is more commonly used because of its time-effectiveness and relative ease. Unfortunately, there is lingering inconsistency on how best to accurately and quickly determine fiber type via IHC and an overall misunderstanding regarding pure fiber type proportions, specifically the abundance of fibers exclusively expressing highly glycolytic MyHC IIX in humans. We therefore 1) present information and data showing the low abundance of pure MyHC IIX muscle fibers in healthy human skeletal muscle and 2) leverage this information to provide straightforward protocols that are informed by human biology and employ inexpensive, easily attainable antibodies for the accurate determination of fiber type.
Source: Journal of Applied Physiology
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Awards: G1420916214, G1838525213, G222091495, Prevention of Skeletal Muscle Adaptations to Traumatic Knee Injury and Surgery, G3637058030, Skeletal muscle contractile dysfunction in heart failure, Single Skeletal Muscle Fiber Mechanics and Myosin Kinetics in Human Aging, G8040286783, Muscle disuse and contractile dysfunction in the elderly, G9000034125, G9000049787, G9000092934, G9000227929, G9000234870, G9000336186, Kentucky Center for Clinical and Translational Science
OpenAlex ID: W2983893214