Author response: Thioredoxin shapes the C. elegans sensory response to Pseudomonas produced nitric oxide
Year: 2018
Type: peer-review
Abstract: Nitric oxide is a colorless gas that contains one nitrogen atom and one oxygen atom. Found at very low levels in the air, this gas is produced by the intense heat of lightning strikes and by combustion engines. Almost all living organisms also produce nitric oxide. In animals, for example, nitric oxide regulates blood pressure and signaling between neurons. However, it was not known if animals could detect nitric oxide in their environment and respond to it. Caenorhabditis elegans is a worm that has been intensively studied in many fields of biology. Unlike most animals, it cannot make nitric oxide. Yet, living in the soil, C. elegans does come into contact with many microbes that can, including the bacterium Pseudomonas aeruginosa. These bacteria can infect and kill C. elegans, and so the worm typically avoids them. Hao, Yang et al. asked whether C. elegans does so by detecting the nitric oxide that these harmful bacteria release into their environment. First, worms were added to a petri dish where a small patch of P. aeruginosa was growing. Consistent with previous results, the worms had all moved away from the bacteria after a few hours. The experiments were then repeated with mutant bacteria that cannot produce nitric oxide. The worms were less likely to avoid these mutant bacteria, suggesting that C. elegans does indeed avoid infection by detecting bacterially produced nitric oxide. Next, using a range of techniques, Hao, Yang et al. showed that C. elegans avoids nitric oxide released into its environment by detecting the gas via a pair of sensory neurons. These neurons require several specific proteins to be able to detect nitric oxide and respond to it. In particular, a protein called Thioredoxin was found to determine the beginning and end of the worm’s sensory response to nitric oxide. All of these proteins are also found in many other animals, and so it is possible that these findings may be relevant to other species too. Further studies are now needed to confirm whether other organisms can sense nitric oxide from their environment and, if so, how their nervous systems equip them to do this.
Source: eLife
Institutions: Harvard University, Massachusetts General Hospital
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,500
OpenAlex ID: W2985296523