Year: 2019
Type: article
Abstract: Endosomal recycling maintains the cell surface abundance of nutrient transporters for nutrient uptake, but how the cell integrates nutrient availability with recycling is less well understood. Here, in studying the recycling of human glutamine transporters ASCT2 (SLC1A5), LAT1 (SLC7A5), SNAT1 (SLC38A1), and SNAT2 (SLC38A2), we establish that following amino acid restriction, the adaptive delivery of SNAT2 to the cell surface relies on retromer, a master conductor of endosomal recycling. Upon complete amino acid starvation or selective glutamine depletion, we establish that retromer expression is upregulated by transcription factor EB (TFEB) and other members of the MiTF/TFE family of transcription factors through association with CLEAR elements in the promoters of the retromer genes VPS35 and VPS26A. TFEB regulation of retromer expression therefore supports adaptive nutrient acquisition through endosomal recycling.
Source: The Journal of Cell Biology
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: hybrid
Awards: G2194756453, G4477519055, G5098779134, G6434476841, G647223558, G7036786280, Defining the role of retromer-like in endolysosomal cargo sorting in health and disease., G7068142517, Analysing the cell biology of SNX10 in endosomal sorting and signaling: implications for osteoclast function in osteopetrosis, Defining the role of retromer in endosomal sorting in health and disease., Defining the role of retromer in endosomal sorting in health and disease., G9000296686
OpenAlex ID: W2986105336