Mouthwashes: an in vitro study of their action on microbial biofilms and cytotoxicity to gingival fibroblasts.
Year: 2018
Type: article
Abstract: This study evaluated the in vitro antibiofilm effect of 5 different commercial mouthwashes (Cepacol Traditional, Colgate Plax Fresh Mint, Listerine Cool Mint, Oral-B Complete, and Sensodyne) on Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans, Escherichia coli, and Pseudomonas aeruginosa. The cytotoxic effect of the mouthwashes on gingival fibroblasts was also analyzed. A colorimetric assay with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was used to investigate the viability of biofilms after 48 hours and gingival fibroblasts after 24 hours. The biofilms were exposed to the mouthwashes for 2 different lengths of time: T1, the time recommended by the manufacturer (30 or 60 seconds); and T2, double the recommended time (60 or 120 seconds). All antiseptic mouthwashes caused a significant reduction of biofilm (P < 0.05) as well as a significant reduction of viable gingival fibroblasts (P < 0.05) with both exposure times (T1 and T2). It can be concluded that the commercial mouthwashes demonstrated effective antibiofilm activity; they were more effective on bacteria than on C albicans. A significant cytotoxic effect on gingival fibroblasts was also observed.
Source: PubMed
Authors: Jonatas Rafael de Oliveira, Kely Karina Belato, Felipe Eduardo de Oliveira, Antonio Olavo Cardoso Jorge, Samira Esteves Afonso Camargo, Luciane Dias de Oliveira
Language: English
Subfield: Periodontics
Field: Dentistry
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2986596712