Work

Dissociation of Nuclear Suspensions from Human Breast Tissues v1

Year: 2018

Type: preprint

Abstract: This protocol can be used to prepare nuclear suspensions from fresh or frozen tissue for single cell DNA or RNA sequencing experiments. For RNA analysis it is recommended to use fresh tissue samples, due to RNA degradation that occurs during freeze- thaw cycles, however for DNA analysis either frozen or fresh tissue can be used. The protocol has been tested on breast tissues, and many frozen tumor tissues including breast, colon prostate and pancreatic samples.

DOI: 10.17504/protocols.io.t3aeqie

Authors: Emi Sei, Shanshan Bai, Nicholas Navin

Language: English

Topic: Single-cell and spatial transcriptomics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

OpenAlex ID: W2990159852

Cites:

Cited by: 5

Related to: 10