Multiplication and Regeneration Potential in Strawberry genotypes using Different In Vitro Culture Methods and Growth Regulators
Year: 2018
Type: article
Abstract: In vitro experiment was conducted at the Plant Tissue Culture Laboratory of the Department ofHorticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt during the period 2015-2016. Effects ofincreasing number of subcultures up to 12th subculture, BA concentrations up to 2.0 mg/l and their interaction onmultiplication rate of four strawberry cultivars were studied. Also, organogenic potential of leaf disc explants fromthree strawberry cultivars were tested using two different media (direct and indirect regeneration medium). Presentedresults showed that the highest multiplication rate in cvs. Kamela and Amega were achieved after the 6th subculturesusing 1.0 mg/l BA, however, the highest multiplication rate in cv. Camarosa was achieved after 7 subcultures using 1.0mg/ BA. The highest multiplication rate in cv. Fortuna was found after 6 subcultures on MS medium+0.5 mg/l BA,however, the lowest multiplication rate in all tested genotypes were after 12th subculture on MS+ 2.0 mg/l BA. Highregeneration capacity was obtained in medium amended with TDZ (direct regeneration medium) compared with 2,4-D+BA (indirect regeneration medium). Fortuna gave the highest regenerated shoots followed by cv. Kamela, then cv.Camarosa (v/v/v). Plantlets were successfully acclimated with 90% survival.
Author: Mohamed, F.H. M.A.A. Hussein
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W2991322381