Year: 2018
Type: article
Abstract: The aim of this study was to set a protocol for the in vitro culture of Curcuma longa L. Sprouted budexplants of C. longa L. were explanted on Murashige and Skoog (MS) medium supplemented with differentconcentrations of 6-benzyladenine (BA) 0, 1, 3, 5, and 7 mg l-1 during six subcultures. The maximum number ofmultiplied shoots (8.60 and 8.70 shoots explant-1), was obtained in the medium containing 7 mg l−1 BA at 4th and5thsubculture with (90 and 100% multiplication frequency) respectively. The optimum BA concentrations for maximumnumber of multiplication varied across subcultures. The 7 mg l-1 BA concentration at the late subculture 4th, 5th and 6thproduce highest values of (number of shoots, longest shoot, number of leaves and fresh weight).The maximum numberof roots (6.38 roots explant-1) was induced from 3 mg l−1IBA. The tallest roots (40.6 and 36.5 cm) were obtained on MSmedium supplemented with 1 mg l−1 NAA and 3 mg l−1 IBA. Also, the heaviest fresh weight was obtained frommedium containing 2 and 3 mg l−1 IBA and all concentrations of NAA. In vitro plantlets immediately acclimatized togreenhouse conditions, showing 100% survival rates in a peat moss and vermiculite (1:3) medium.
Author: Koriesh, E. M Hefni M. M.
Institution: Suez Canal University
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W2991353676