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Anti-apoptotic factor Birc3 is up-regulated by ELL2 knockdown and stimulates proliferation in LNCaP cells.

Year: 2019
Type: article
Abstract: ELL2 is a potential tumor suppressor in prostate cancer. ELL2 knockout in mice induced mPIN, the putative precursor of prostate cancer and ELL2 knockdown enhanced proliferation in cultured prostate cancer cells. To explore the mechanism of ELL2 action in prostate cancer, we investigated the role of Birc3, an apoptosis inhibitor, in prostate cancer cells and the regulation of its expression by ELL2. ELL2 knockdown enhanced Birc3 expression in LNCaP and C4-2 cell line models. BrdU assay showed that Birc3 knockdown inhibited proliferation, ELL2 knockdown enhanced proliferation, and Birc3 knockdown counteracted ELL2 knockdown-induced proliferation in LNCaP cells. Trypan blue assay suggested that Birc3 knockout did not induce cell death in LNCaP cells. These findings suggested that Birc3 is a downstream gene of ELL2 and may play a role in driving prostate cancer proliferation.
Authors: Zhi Wang, Mingming Zhong, Qiong Song, Laura E Pascal, Zhenyu Yang, Zeyu Wu, Ke Wang, Zhou Wang
Institutions: Central South University, University of Pittsburgh, Xiangya Hospital Central South University, Guangxi Medical University, First Affiliated Hospital of Xi'an Jiaotong University, UPMC Hillman Cancer Center, University of Pittsburgh Medical Center
Language: English
Field: Medicine
Open Access status: green
OpenAlex ID: W2991687802
FWCI: 0.738
Cites: 39
Cited by: 9
Related to: 10