Year: 2019
Type: conference-paper
Abstract: This work reports Tobacco mosaic virus (TMV)-based assembly of glucose oxidase (GOx) electrodes for the development of advanced enzymatic biofuel cells (EBCs). Cysteine-modified TMV (TMV1cys), self-assembled on Au electrodes, has served as a template for augmenting the GOx immobilization density via a robust chemical conjugation route. Enhanced enzymatic activity of the TMV1cys/GOx electrodes have been confirmed via colorimetric assay, and the electrochemical transduction of the catalytic reactions via mediated electron transfer resulted in a ~25-fold increase in electrochemical current compared to previous work using a similar strategy. This enhancement is attributed to the on-chip bioconjugation strategy presented in this work, leveraging the robust and high density self-assembly of TMV1cys on Au surface. The performance of an EBC consisting of TMV-assembled enzyme electrodes is reported for the first time, generating a maximum power density of 860 nW/cm2.
Institution: University of Maryland, College Park
Language: English
Subfield: Electrical and Electronic Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: diamond
Funder: Army Research Office
Awards: G3141641755, G3249006235, G7452299184
OpenAlex ID: W2992313415