3D Nanofibrillar Cellulose hydrogel platform for long-term human islet culture v1
Year: 2018
Type: preprint
Abstract: It remains a challenge to maintain human isletsex vivoto provide a functional and sufficient source for clinical islet transplantation and anin vitromodel for drug screening or basic biological studies. With a conventional culture method, islets lost their functionality and mass rapidly upon they were isolated from the native environment, which provides both mechanical and biological supports. By merging the advantage of transwell cultures and the mechanical properties provided by the wood-derived nanofibrillar cellulose (NFC) hydrogel, we developed a new isletex vivoplatform, which can preserve human islets up to 83 days with intact morphology similar to freshly isolated counterparts. Importantly, these long-term cultured human islets are still potent and can ameliorate hyperglycemia in diabetic mice post-transplantation. The platform is designed to beuser friendlyand easily adapted to help scientists overcome the difficulties in islet cultures. Furthermore, the application of this platform aims to provide a sufficient window for pre-transplantation islet assessment, a biobanking opportunity of human islets, and anin vitromodel for human islet studies in drug-screening, stem cell-derived islet (beta)-like organoids maturation, and basic human islet biology.
Authors: Yi-Ju Chen, Ben Z. Stanger
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2993079100