Work

Randomness and optimality in enhanced DNA ligation with crowding effects

Year: 2020

Type: article

Abstract: This paper studies enzymatic DNA ligation in a crowded polymer solution. The authors establish qPCR-based statistical analysis of ligation randomness and show that a coexisting polymer optimizes the ligation efficiency, depending on the intermolecular attractions of DNA and freely available enzymes.

Source: Physical Review Research

DOI: 10.1103/physrevresearch.2.013360

Authors: Takaharu Y. Shiraki, Ken-ichiro Kamei, Yusuke T. Maeda

Institutions: Kyushu University, Kyoto University

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,625

Funders: Human Frontier Science Program, Ministry of Education, Culture, Sports, Science and Technology, Japan Society for the Promotion of Science