Year: 2019
Type: article
Abstract: Fluctuation X-ray scattering (FXS) is a biophysical technique that overcomes the low datato-parameter ratios encountered in traditional X-ray scattering methods used for studying noncrystalline samples [1].In an FXS experiment solution scattering data are collected from particles in solution using ultrashort X-rays of pulse lengths shorter than the rotational diffusion time of the particles.The resulting data contains angularly varying information that yields structures with a greater level of detail than those obtained using tradition SAXS [2].After successful application of FXS to single-particle data [3], we have recently demonstrated the experimental feasibility of this technique on data from an ensemble of PBCV-1 virus particles in solution [4,5].We found that by using advanced noise-filtering methods the required number of images to obtain decent correlation data is far lower than originally expected, thereby reducing the required data collection time to less than a few minutes.In this talk I will outline the data processing techniques for analysis of FXS data, and present an assessment of the effect of concentration and various sources of noise on experimental data.
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W2997564833