Work

Structure of the Fab-Labeled “Breathing” State of Native Poliovirus

Year: 2012

Type: article

Abstract: At 37°C, the structure of poliovirus is dynamic, and internal polypeptides VP4 and N terminus of VP1 (residues 1 to 53) externalize reversibly. An Fab fragment of a monospecific antibody, which binds to residues 39 to 55 of VP1, was utilized to locate the N termini of VP1 in native (160S) particles in this "breathing" state. Fab and virus were mixed and imaged via cryogenic electron microscopy. The resulting reconstruction showed the capsid expands similarly to the irreversibly altered cell entry intermediate (135S) particle, but the N terminus of VP1 is located near the 2-fold axes, instead of the "propeller tip" as in 135S particles.

Source: Journal of Virology

DOI: 10.1128/jvi.05990-11

Authors: Jun Lin, Lily Y. Lee, Merja Roivainen, David J. Filman, James M. Hogle, David M. Belnap

Institutions: Brigham Young University, Harvard University, Finnish Institute for Health and Welfare

Language: English

Topic: Viral Infections and Immunology Research

Subfield: Cardiology and Cardiovascular Medicine

Field: Medicine

Domain: Health Sciences

Open Access status: bronze

Funder: National Institute of Allergy and Infectious Diseases

Awards: Temperature-dependent, Intermediate Particles from the Entry of Picornaviruses in, Poliovirus Cell Entry Pathways