Infuence of chemically synthesized copper nanoparticles and cupric ions on oxalate oxidation system in germinating Sorghum grain
Year: 2020
Type: article
Abstract: We have earlier reported the effects of chemically synthesized copper nanoparticles (CuNPs) on oxalate oxidase (OxOx) activity, extracted from the shoot tissue of germinating grain sorghum i.e. in vitro . Here, we tried to study this effect in vivo and compare it with those of Cu2+. We describe herein, characterization of CuNPs and their effects on oxalate oxidation system i.e. OxOx activity, total oxalate and H2O2 content in vivo i.e. in shoot tissues/leaves of germinating grain Sorghum ( Sorghum vulgare L). To achieve it, grain sorghum seeds were grown up to 10 days in laboratory, irrigated with Hoagland’s solution containing either CuNPs (1.0 ppm) or Cu2+ (1.0 ppm) after 4 days of germination. Control were irrigated with Hoagland solution only. The shoot/leaves of the seedling plants were harvested at 4, 6, 8 and 10 day of germination and analysed quantitatively for OxOx activity, soluble protein, H2O2 and total oxalate. The growth of the Sorghum seedling plants supplemented with CuNPs and Cu2+ was decreased significantly (P <0.1) at all growth stages compared to control. This inhibitory effect of CuNPs was higher than Cu2+. CuNPs decreased the activity of OxOx but Cu2+ had no effect at day 10. Both CuNPs and Cu2+ decreased the specific activity of OxOx and H2O2 content but increased total oxalate content at day 10. The decrease in H2O2 content in both CuNPs and Cu2+ supplemented shoot tissues with concomitant increase in oxalate content confirmed the decreased activity of OxOx in CuNPs and Cu2+ supplemented seedling plants.
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W2998761947