HYPERsol: High-Quality Data from Archival FFPE Tissue for Clinical Proteomics
Year: 2020
Type: article
Abstract: Massive formalin-fixed, paraffin-embedded (FFPE) tissue archives exist worldwide, representing an invaluable resource for clinical proteomics research. However, current protocols for FFPE proteomics lack standardization, efficiency, reproducibility, and scalability. Here we present high-yield protein extraction and recovery by direct solubilization (HYPERsol), an optimized workflow using ultrasonication and S-Trap sample processing that enables proteome coverage and quantification from FFPE samples comparable to that achieved from flash-frozen tissue (average R = 0.936). When applied to archival samples, HYPERsol resulted in high-quality data from FFPE specimens in storage for up to 17 years, and may enable the discovery of new immunohistochemical markers.
Source: Journal of Proteome Research
Institutions: University of Pennsylvania, Cold Spring Harbor Laboratory
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
Awards: G1375395185, G1564983540, Structural biology & molecular biophysics training program, G2476700337, Role of Histone and Histone-like Mutations in the Oncogenesis of Human Cancers, G3457235877, G666505512, G830265429, NRSA Training Core, Physician Scientist Training in Clinical Oncology, CSHL Cancer Center Support Grant
OpenAlex ID: W2998898017