Work

A ‘light-up’ intercalator displacement assay for detection of triplex DNA stabilizers

Year: 2020

Type: article

Abstract: Here, we developed a coralyne-based, 'light-up' intercalator displacement assay to identify molecular stabilizers of triplex DNA using a sequence from a chromosomal breakpoint hotspot in the human c-MYC oncogene. Its potential to identify triplex DNA ligands was demonstrated using BePI and doxorubicin. Identification of triplex-interacting ligands may allow the regulation of genetic instability in human genomes.

Source: Chemical Communications

DOI: 10.1039/c9cc08817b

Authors: Imee M. A. del Mundo, Eun Jeong Cho, Kevin N. Dalby, Karen M. Vasquez

Institution: The University of Texas at Austin

Language: English

Topic: DNA and Nucleic Acid Chemistry

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Welch Foundation, Cancer Prevention and Research Institute of Texas, National Institutes of Health, National Cancer Institute

Awards: Targeted Therapeutic Drug Discovery & Development Program, G52711424, Molecularly-Targeted Approaches to Cancer Therapeutics, Diagnostics and Prevention, Repair of Genome Destabilizing DNA Structures