Comparative analysis of enteroendocrine cells and their hormones between mouse intestinal organoids and native tissues
Year: 2020
Type: article
Abstract: Endocrine cells in the gastrointestinal tract secrete multiple hormones to maintain homeostasis in the body. In the present study, we generated intestinal organoids from the duodenum, jejunum, and ileum of Neurogenin 3 (Ngn3)-EGFP mice and examined how enteroendocrine cells (EECs) within organoid cultures resemble native epithelial cells in the gut. Transcriptome analysis of EGFP-positive cells from Ngn3-EGFP organoids showed gene expression pattern comparable to EECs in vivo. We also compared mRNAs of five major hormones, namely, ghrelin (Ghrl), cholecystokinin (Cck), Gip, secretin (Sct), and glucagon (Gcg) in organoids and small intestine along the longitudinal axis and found that expression patterns of these hormones in organoids were similar to those in native tissues. These findings suggest that an intestinal organoid culture system can be utilized as a suitable model to study enteroendocrine cell functions in vitro.
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: green
Awards: Development of a Human Taste-Small Intestine-Brain Correlation Model applying for Nutritional Physiology, Development of new technology for taste cell omics: exploring molecular logics of high calorie sensing and taste signal transduction, Molecular physiological basis of modulation and repair by oxidative stress in oral/gut/ brain chemosensory-endocrine interaction for dietary regulation.
OpenAlex ID: W2999003276