Dual-initiation promoters with intertwined canonical and TCT/TOP transcription start sites diversify transcript processing
Year: 2020
Type: article
Abstract: Variations in transcription start site (TSS) selection reflect diversity of preinitiation complexes and can impact on post-transcriptional RNA fates. Most metazoan polymerase II-transcribed genes carry canonical initiation with pyrimidine/purine (YR) dinucleotide, while translation machinery-associated genes carry polypyrimidine initiator (5’-TOP or TCT). By addressing the developmental regulation of TSS selection in zebrafish we uncovered a class of dual-initiation promoters in thousands of genes, including snoRNA host genes. 5’-TOP/TCT initiation is intertwined with canonical initiation and used divergently in hundreds of dual-initiation promoters during maternal to zygotic transition. Dual-initiation in snoRNA host genes selectively generates host and snoRNA with often different spatio-temporal expression. Dual-initiation promoters are pervasive in human and fruit fly, reflecting evolutionary conservation. We propose that dual-initiation on shared promoters represents a composite promoter architecture, which can function both coordinately and divergently to diversify RNAs.
Source: Nature Communications
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $5,380
Awards: G2328893653, Promoter-associated histone modifications and establishment of the developmental gene expression program during early embryogenesis, G4889237663, G5201049165, G5934299761, The core promoter: an unexplored regulatory level of transcription during vertebrate development., Promoter-associated histone modifications and establishment of the developmental gene expression programme during early embryogenesis, G950397263
OpenAlex ID: W2999555406