Author Correction: A Recombinant Newcastle Disease Virus (NDV) Expressing S Protein of Infectious Bronchitis Virus (IBV) Protects Chickens against IBV and NDV
Year: 2020
Type: erratum
Abstract: This Article contains errors in the Methods under subheading 'IBV protection experiment 2' . "A total of twenty 4-week-old SPF chickens were divided into four groups of five each. Five chickens of groups one and two were inoculated with 10 7 EID 50 of rNDV and rNDV/codon optimized-S, respectively, via oculanasal route. Five chickens of group three were inoculated with 10 recommended doses of a commercial live attenuated Mass-type IBV vaccine via oculanasal route and chickens of group four were inoculated with PBS. " should read: "A total of fifteen 4-week-old SPF chickens were divided into three groups of five each. Five chickens of groups one and two were inoculated with 10 7 EID 50 of rNDV/codon optimized-S and 10 recommended doses of a commercial live attenuated Mass-type IBV vaccine via oculanasal route, respectively. Chickens of group three were inoculated with PBS. Samples collected from five non-vaccinated SPF chickens involved in another IBV protection study also were used as control. " Furthermore, within the Methods under subheading 'Quantitative reverse transcription-polymerase chain reaction (RT-qPCR)' "Forty cycles of PCR at 95 C for 10 s (denaturation), 58 C for 20 s (annealing), and 72 C for 30 s (elongation) followed by melting curve analysis that consisted of 95 C for 5 s and 65 C for 60 s. " should read: "The thermal conditions of 50 C for 10 min and 95 C for 10 min, then forty-five cycles of PCR at 95 C for 10 s (denaturation), 58 C for 20 s (annealing) followed by 95 C for 5 s and melting curve analysis that consisted of 65 C to 95 C for 5 s was carried out to amplify the 150 nt -N gene fragments. "
Source: Scientific Reports
Language: English
Subfield: Animal Science and Zoology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,870
OpenAlex ID: W2999775171