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Two-photon microscope using a fiber-based approach for supercontinuum generation and light delivery to a small-footprint optical head

Year: 2020
Type: article
Abstract: In this Letter, we report a low-cost, portable, two-photon excitation fluorescence microscopy imager that uses a fiber-based approach for both femtosecond supercontinuum (SC) generation and light delivery to the optical head. The SC generation is based on a tapered polarization-maintaining photonic crystal fiber that uses pre-chirped femtosecond narrowband pulses to generate a coherent SC spectrum with a bandwidth of approximately 300 nm. Using this approach, high-power, near-transform-limited, wavelength-selectable SC pulses are generated and directly delivered to the imaging optical head. Preliminary testing of this imager on brain slices is presented, demonstrating a high signal-to-noise ratio and sub-cellular imaging capabilities to a depth of approximately 200 µm. These results demonstrate the suitability of the technology for ex vivo and potentially in vivo cellular-level biomedical imaging applications.
DOI: 10.1364/ol.381571
Authors: Youbo Zhao, Gopi Maguluri, R. Daniel Ferguson, Haohua Tu, Kush Paul, Stephen A. Boppart, Daniel A. Llano, Nicusor Iftimia
Language: English
Open Access status: green
Funders: National Science Foundation, National Institutes of Health, National Institute of Mental Health, National Cancer Institute, National Institute on Deafness and Other Communication Disorders, National Institute of Biomedical Imaging and Bioengineering
Awards: G150082591, An upright multiphoton microscope for biomedical research applications, G3516691268, G465651580, Portable optogenetics/two-photon imaging instrument for in vivo brain studies, G6919057395, G7875776836, G802834745, Handheld Two-Photon Microscopy Imager, EAGER: Optical Molecular Imaging of Opioid Distribution and its Metabolic Effects in the Brain
OpenAlex ID: W2999790250
FWCI: 0.59
Cites: 27
Cited by: 7
Related to: 10