Work

Small RNA In Situ Hybridizations on Sections of Arabidopsis Embryos

Year: 2020

Type: book-chapter

Abstract: Small RNAs mediate posttranscriptional gene silencing in plants and animals. This often occurs in specific cell or tissue types and can be necessary for their differentiation. Determining small RNA (sRNA) localization patterns at cellular resolution can therefore provide information on the corresponding gene regulatory processes they are involved in. Recent improvements with in situ hybridization methods have allowed them to be applied to sRNAs. Here we describe an in situ hybridization protocol to detect sRNAs from sections of early staged Arabidopsis thaliana (Arabidopsis) embryos.

Source: Methods in molecular biology

DOI: 10.1007/978-1-0716-0342-0_7

Authors: Katalin Páldi, Magdalena Mosiolek, Michael D. Nodine

Institutions: Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna Biocenter

Language: English

Topic: Plant Molecular Biology Research

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: hybrid

Funders: European Commission, H2020 European Research Council

Awards: G434337789, Small RNA regulation of the body plan and epigenome in Arabidopsis embryos