MOESM3 of PfCap380 as a marker for Plasmodium falciparum oocyst development in vivo and in vitro
Year: 2018
Type: article
Abstract: Additional file 3: Figure S3. Negative control IFA on in vivo and in vitro oocysts. IFA were performed as described except no primary antisera was used to test secondary antibodies for non-specific binding. Secondary antibodies were used to label midgut oocysts (A) or in vitro oocysts (B) and show expression of GFP in green and DAPI nuclear staining in blue. The merged image of the three separate channels is shown. DIC images were taken for in vitro but not in vivo oocysts due to challenges in imaging midgut tissue. Scale bars = 10 Îźm. For midgut oocysts, negative control antibodies used were Alexa Fluor 594 anti-rabbit for Cap380 and Alexa Fluor 647 anti-mouse for CSP. For in vitro oocysts, negative control antibodies used were Alexa Fluor 594 anti-rabbit for Cap380 and Alexa Fluor 594 anti-mouse for CSP.
Source: Figshare
Authors: Itsara, Leslie, Zhou, Yaxian, Do, Julie, Dungel, Samrita, Fishbaugher, Matthew, Betz, Will, Nguyen, Thao, Navarro, Mary, Flannery, Erika, Vaughan, Ashley, Kappe, Stefan, Ghosh, Anil
Language: English
Topic: Malaria Research and Control
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W6958439585