Year: 2020
Type: dissertation
Abstract: Daylilies (Hemerocallis spp.) are ornamental flowering plants with over 80,000 unique registered cultivars. Traditionally propagated through division, multiplication rates range from 1-20 divisions per season depending on cultivar, leading to a delay of up to 17 years for newly registered cultivars to reach a marketable level of plant material. Micropropagation offers an alternative approach that can dramatically increase multiplication rates and is used extensively for the species. However, protocols have only been developed for a selected number of genotypes and many protocols go through a callus phase introducing concern over somaclonal variation. The objective of this study was to evaluate the multiplication stage of micropropagation to optimize a protocol that minimizes unwanted callus with some degree of compatibility among different cultivars. With the use of multiple cytokinins in a liquid medium, a protocol has been established and successfully used to bring several cultivars into culture.
Authors: J. L. Callaghan, Max Jones
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W3003967914