Year: 2002
Type: article
Abstract: Aims: To examine the differential expression of collagens in the rabbit sclera at several stages of early postnatal development from 1 day to 4 weeks.Materials and methods: Total RNA was extracted from the sclera of 2 groups of rabbits at each time point and real-time polymerase chain reaction analysis was employed. The amount of message for each collagen gene was compared with the corresponding level for 1 day mRNA. Histological examination was performed for sclera at 1 day and 4 weeks.Results: Real time polymerase chain reaction allows the quantification of message level. The mRNA level for collagen a1 (I), a2 (I), and a1 (III) were highest 1 day after birth and declined gradually during the observation period. Collagen a2 (XI) demonstrated a relatively similar trend of expression. Histological analysis showed that scleral fibroblast density was greatest at 1 day.Conclusion: Collagens play a crucial role in active tissue construction during the postnatal developmental period and changes in the level of transcription of these genes could affect the overall structure of the sclera.
Institution: Singapore Eye Research Institute
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: diamond
Award: G3181593711
OpenAlex ID: W3005071978