Evaluation of PD-1 blockade using a multicellular tumor spheroid model.
Year: 2019
Type: article
Abstract: models to evaluate the effects of PD-1 blockade has restricted use of PD-1 inhibitors. Here, we established spheroids from a human gastric cell line to evaluate the cytotoxicity of the PD-1 blockade. Mature spheroids were formed 72 h after seeding tumor cells into ultra-low attachment microplates. We induced PD-L1 expression of spheroids using IFN-γ and TNF-α. Confocal images showed that PD-1 blockade enhanced T-cell cytotoxicity against PD-L1-expressing spheroids. Mechanistic studies revealed that PD-1 blockade increased expression of multiple chemokines. Altogether, our study demonstrates that tumor spheroids provide a novel platform to evaluate the effects of PD-1 blockade and might be further utilized to develop promising combination therapies.
Source: Europe PMC (PubMed Central)
Authors: Shujuan Zhou, Mei Zhu, Fanyan Meng, Jie Shao, Qiuping Xu, Jia Wei, Baorui Liu
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W3005097947