miR-193-3p ameliorates bone resorption in ovariectomized mice by blocking NFATc1 signaling.
Year: 2019
Type: article
Abstract: BACKGROUND: Nuclear factor of activated T cells, cytoplasmic 1 (NFATc1) as a key transcription factor contributes to osteoclast differentiation and bone resorption. However, the post-transcriptional mechanisms of microRNAs (miRNAs) targeted to NFATc1 have not been completely clarified in postmenopausal osteoporosis (PMO). In our study, we aimed to investigate the role of miR-193-3p in ovariectomy (OVX)-induced bone loss by regulating the NFATc1 pathway. METHODS: Female C57BL/6J mice underwent sham or OVX operation. Injection of Agomir-Control or Agomir-miR-193-3p was performed in OVX mice. Serum, urine and tibia were collected for experimental measurements, including biochemical markers, RT-qPCR and western blotting assays. RESULTS: dyshomeostasis and bone loss by inhibiting the expression of NFATc1 and its downstream targets of osteoclast-specific genes, Ctsk, TRAP and Car2. CONCLUSION: Overexpression of miR-193-3p had an osteoprotective effect in OVX mice by suppressing NFATc1 pathways.
Source: PubMed Central
Authors: Xiuhua Li, Limin Yang, Zhanpeng Guo
Institution: Jinzhou Medical University
Language: English
Topic: Bone Metabolism and Diseases
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W3006721009