Microbial Colonization of Germ‐Free Mice Restores Neointimal Hyperplasia Development After Arterial Injury
Year: 2020
Type: article
Abstract: Background The potential role of the gut microbiome in cardiovascular diseases is increasingly evident. Arterial restenosis attributable to neointimal hyperplasia after cardiovascular procedures such as balloon angioplasty, stenting, and bypass surgery is a common cause of treatment failure, yet whether gut microbiota participate in the development of neointimal hyperplasia remains largely unknown. Methods and Results We performed fecal microbial transplantation from conventionally raised male C57BL/6 mice to age-, sex-, and strain-matched germ-free mice. Five weeks after inoculation, all mice underwent unilateral carotid ligation. Neointimal hyperplasia development was quantified after 4 weeks. Conventionally raised and germ-free cohorts served as comparison groups. Conclusions Germ-free mice have significantly attenuated neointimal hyperplasia development compared with conventionally raised mice. The arterial remodeling response is restored by fecal transplantation. Our results describe a causative role of gut microbiota in contributing to the pathogenesis of neointimal hyperplasia.
Language: English
Topic: Gut microbiota and health
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,750
Funders: National Institutes of Health, National Heart, Lung, and Blood Institute, National Institute of Nursing Research, National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases, National Center for Advancing Translational Sciences, Eunice Kennedy Shriver National Institute of Child Health and Human Development
Awards: The Role of Gut Microbiota in Neointimal Hyperplasia After Vascular Injury, Inter-kingdom communication between intestinal microbiota and mammalian host via cyclic dinucleotides - Resubmission 01, Azithromycin and the airway microbiome in asthma, Gut microbiota, short chain fatty acids, and adiposity across the epidemiologic transition, Northwestern University Clinical and Translational Science Institute (NUCATS), Dietary fat effects on gut microbes, host immune state and experimental colitis, Digestive Health and Diseases (DHD) Training Grant, Human Microbiome and Perinatal Depression, Vascular Surgery Scientist Training Program, Diet induced obesity from gut microbial disruption of host metabolic networks, The Preterm Infant Microbiome: Biological, Behavioral and Health Outcomes at 2 and 4 years of Age, Diurnal Oscillations in Gut Microbes and Host Immunity in Health and Obesity, Prevention of Lower Urinary Tract Symptoms in Women: Bladder Health Scientific and Data Coordinating Center (PLUS-SDCC) (U01), Center for Interdisciplinary Study of Inflammatory Intestinal DIsorders (C-IID), Gut pathogen virulence and its therapeutic modulation during surgical injury
OpenAlex ID: W3008439494