Work

Inhibitory effect of miR-145 on RPE cell proliferation.

Year: 2016

Type: article

Abstract: OBJECTIVE: This study aims to explore the impact of micro RNA miR-145 on retinal pigment epithelial cell proliferation and apoptosis. METHODS: A stable culture and passage system of hPNE cells was first established, and its migration ability was determined. Then, miR-145 lentiviral vectors were constructed to transfect hPRE cells. Thereafter, hRPE cell proliferation was detected by MTT assay after they were transfected by lentivirus, cell cycle was analyzed by flow cytometry, and apoptosis was detected by Annexin V/PI double staining immunofluorescence. RESULTS: phase was inhibited; thus, apoptosis rate increased. CONCLUSION: MiR-145 can slow down retinal pigment epithelial cell proliferation and increase their apoptosis rate. This has a certain therapeutic potential for diseases caused by RPE cell proliferation such as PVR.

Source: PubMed Central

Authors: Ken Zhao, Zhen Chen, Xu-Dong Lv, Gang Dong, Huan Xia

Institutions: Hubei Polytechnic University, Daye People's Hospital, Renmin Hospital of Wuhan University, Xianning Central Hospital, Hubei University of Science and Technology

Language: English

Topic: Retinal Diseases and Treatments

Subfield: Ophthalmology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W3010592338

FWCI:

Cites: 20

Cited by: 1

Related to: 10