Year: 2019
Type: preprint
Abstract: Prepare four wells of the cell line of interest by EDTA split (Basic Protocol 1). NoteFor at least the first time performing this protocol, include positive and negative control cells in order to compare with cells of interest.2 1 to 2 days after plating, follow Basic Protocol 2 (steps 6 to 9) for two of the wells in order to transfect with the pBI-MCS-EGFP plasmid. 2.1For each transfection, add 100 µL of Opti-MEM and 3 µg of total DNA to one 1.5-ml microcentrifuge tube and vortex for 00:00:02 to 00:00:03 .In a second tube, combine 100 µL of Opti-MEM and 10 µL of Lipofectamine Stem reagent, and vortex for
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W3010728968