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GENOTYPING OF iPSCS WITH GENE INSERTIONS (Support Protocol 1) v1

Year: 2019

Type: preprint

Abstract: This protocol will use genomic DNA isolated from the purified iPSC clones with CLYBL or AAVS1 gene insertion to determine if integration of the transgene has occurred correctly, in a heterozygous or homozygous fashion, and if the floxed selection genes are present. Primer sequences, amplicon sizes, PCR mix composition, and thermal conditions are included in the steps.

DOI: 10.17504/protocols.io.5u9g6z6

Authors: Michael S. Fernandopulle, Ryan Prestil, Christopher Grunseich, Chao Wang, Li Gan, Michael E. Ward

Institutions: National Institutes of Health, Gladstone Institutes, National Institute of Neurological Disorders and Stroke

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

OpenAlex ID: W3010833508

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