Year: 2020
Type: article
Abstract: BACKGROUND: Lipopolysaccharide (LPS) is a classic immune stimulus. LPS combined with positron emission tomography (PET) 18 kDa translocator protein (TSPO) brain imaging provides a robust human laboratory model to study neuroimmune signaling. To evaluate optimal analysis of these data, this work compared the sensitivity of six quantification approaches. METHODS: C]PBR28 data from healthy volunteers (N = 8) were collected before and 3 h after LPS challenge (1.0 ng/kg IV). Quantification approaches included total volume of distribution estimated with two tissue, and two tissue plus irreversible uptake in whole blood, compartment models (2TCM and 2TCM-1k, respectively) and multilinear analysis-1 (MA-1); binding potential estimated with simultaneous estimation (SIME); standardized uptake values (SUV); and SUV ratio (SUVR). RESULTS: = 0.56-0.89, ps <. 05), whereas SUV and SUVR did not. CONCLUSION: These findings highlight the importance of incorporating AIF measurements to quantify LPS-TSPO studies.
Source: EJNMMI Research
Institutions: Yale University, Copenhagen University Hospital
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,790
Funders: U.S. Department of Veterans Affairs, Yale University, National Alliance for Research on Schizophrenia and Depression, National Institutes of Health, National Center for PTSD, U.S. Department of Veterans Affairs, National Institute on Alcohol Abuse and Alcoholism, National Institute of Mental Health, National Institute on Drug Abuse, National Center for Advancing Translational Sciences
OpenAlex ID: W3011008348