Epidemiological survey of serum titers from adults against various Gram-negative bacterial V-antigens
Year: 2020
Type: article
Study design: Observational Study
Abstract: The V-antigen, a virulence-associated protein, was first identified in Yersinia pestis more than half a century ago. Since then, other V-antigen homologs and orthologs have been discovered and are now considered as critical molecules for the toxic effects mediated by the type III secretion system during infections caused by various pathogenic Gram-negative bacteria. After purifying recombinant V-antigen proteins, including PcrV from Pseudomonas aeruginosa, LcrV from Yersinia, LssV from Photorhabdus luminescens, AcrV from Aeromonas salmonicida, and VcrV from Vibrio parahaemolyticus, we developed an enzyme-linked immunoabsorbent assay to measure titers against each V-antigen in sera collected from 186 adult volunteers. Different titer-specific correlation levels were determined for the five V-antigens. The anti-LcrV and anti-AcrV titers shared the highest correlation with each other with a correlation coefficient of 0.84. The next highest correlation coefficient was between anti-AcrV and anti-VcrV titers at 0.79, while the lowest correlation was found between anti-LcrV and anti-VcrV titers, which were still higher than 0.7. Sera from mice immunized with one of the five recombinant V-antigens displayed cross-antigenicity with some of the other four V-antigens, supporting the results from the human sera. Thus, the serum anti-V-antigen titer measurement system may be used for epidemiological investigations of various pathogenic Gram-negative bacteria.
Source: PLoS ONE
Institutions: Kyoto Prefectural University of Medicine, Kyorin University
Language: English
Subfield: Endocrinology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,695
Awards: G1266447402, Development of a Novel Immunotherapy for Pseudomonas aeruginosa Lung Injury, Development of a novel vaccine therapy using Pseudomonas aeruginosa-derived exosomes, G3168875541, Single-domian antibody against the virulence associated with the type III secretion system of gram-negative bacteria, G3768973757, G4374581737, An epidemiological investigation of serum titers of anti-Pseudomonas aeruginosa virulent antigens and a trial of a specific anti-Pseuomonal gamma-globulin product, G5316758738, Serological immune response against major secretory proteins expressed by Pseudomonas aeruginosa upon intratracheal infection, Preclinical trial of bacteriophage therapy against severe Pseudomonas aeruginosa pnneumoina, Epidemiological study of serum titers against V-antigens of gram-negative bacteria, G7632718820, G7694205865, G817290567, G821146753, Proteomic analysis of lung epithelial lining fluids collected from the patients suffering from acute lung injury., Development of Combined Antibody Therapy Using DDS for MRSA Pneumonia
OpenAlex ID: W3011526046