[Protective Effect of Acupuncture Serum Derived from Acute Convulsion Rats on Cultured Hip-pocampal Neurons with Seizure-like Discharges by Regulating Expression of Endoplasmic Reticulum Stress-inducible Molecular Chaperones].
Year: 2017
Type: article
Abstract: OBJECTIVE: To observe the effect of acupuncture serum on the number of apoptosis of the cultured hippocampal neurons with seizure-like discharges and the expression levels of endoplasmic reticulum stress-inducible molecular chaperones glucose-regulated protein 78 (GRP 78), C/EBP homologous protein (CHOP) and cysteine protease protein-12(Caspase-12), so as to reveal its protective mechanism on seizure-induced injury of hippocampal neurons. METHODS: -free extracellular fluid for 3 h and returned to DMEM/F 12(1:1) plus non-acupuncture serum (9:1) for non-acupuncture serum group. The cell apoptosis was assessed at 2, 12 and 48 h after application of acupuncture serum or non-acupuncture serum by using TUNEL method and the expression levels of GRP 78, CHOP and Caspase-12 proteins of the cultured cells at the 3 time-points detected using Western blot. RESULTS: >0.05). CONCLUSIONS: Acupuncture serum can significantly reduce apoptosis of the cultured hippocampal neurons, which may be related to its effects in increasing the expression of GRP 78 protein and down-regulating the expression of CHOP and Caspase-12 proteins, suggesting an important role of acupuncture serum in maintaining the stability of the neuronal endoplasmic reticulum.
Source: PubMed
Authors: Hui Zhang, Fan Yang, Xu Wu, De-Kai Shen, Hao Chen, Sheng-Bing Wu, Wen-Ping Ang, Xiao-Ge Song, Ming-Yue Xie
Language: Chinese
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W3011538953