Get started
Work

Generation of twelve induced pluripotent stem cell lines from two healthy controls and two patients with sporadic amyotrophic lateral sclerosis

Year: 2020
Type: article
Abstract: The majority of amyotrophic lateral sclerosis are sporadic (sALS) with no familial history or known genetic association, therefore a large cohort of disease models are required to identify common mechanisms or to test therapeutic interventions. Here we generated twelve induced pluripotent stem cell (iPSC) lines from human dermal fibroblasts of two healthy individuals and two sALS patients lacking common ALS mutations, using non-integrational Sendai virus expressing reprogramming factors OCT3/4, KLF4, SOX2 and c-MYC. The iPSC lines highly expressed pluripotency markers could be spontaneously differentiated into three embryonic germ layers, with no gross chromosomal aberrations or specific copy number variations.
DOI: 10.1016/j.scr.2020.101752
Authors: Meimei Yang, Min Liu, Yicheng Ding, Alice Vajda, Jun Ma, Huixian Cui, Timothy O'Brien, David Henshall, Orla Hardiman, Sanbing Shen
Institutions: Royal College of Surgeons in Ireland, Science Foundation Ireland, National University of Ireland, Ollscoil na Gaillimhe – University of Galway, Hebei Normal University, Trinity College Dublin, Hebei Medical University
Language: English
Open Access status: gold
Estimated APC paid: $2,100
OpenAlex ID: W3011619101
FWCI: 0.08
Cites: 5
Cited by: 3
Related to: 10